This lists songs that hit number one on the charts in the US during 1965. If a song spent nonconsecutive weeks at number one, the number of weeks for each occurrence at the top is shown in parenthesis.
| Date | Artist - Title | Weeks at #1 |
| December 20 1964 – January 9 1965 | Beatles - I Feel Fine | 3 |
| January 10 – 16 1965 | Supremes - Come See About Me | 2 (1) |
| January 17 – 30 1965 | Petula Clark - Downtown | 2 |
| January 31 – February 13 1965 | Righteous Brothers - You've Lost That Lovin' Feelin' | 2 |
| February 14 – 27 1965 | Gary Lewis and The Playboys - This Diamond Ring | 2 |
| February 28 – March 6 1965 | Temptations - My Girl | 1 |
| March 7 – 20 1965 | Beatles - Eight Days A Week | 2 |
| March 21 – April 3 1965 | Supremes - Stop! In The Name of Love | 2 |
| April 4 – 17 1965 | Freddie and The Dreamers - I'm Telling You Now | 2 |
| April 18 – 24 1965 | Wayne Fontana and the Mindbenders - Game of Love | 1 |
| April 25 – May 15 1965 | Herman's Hermits - Mrs. Brown, You've Got A Lovely Daughter | 3 |
| May 16 – 22 1965 | Beatles - Ticket to Ride | 1 |
| May 23 – June 5 1965 | Beach Boys - Help Me, Rhonda | 2 |
| June 6 – 12 1965 | Supremes - Back In My Arms Again | 1 |
| June 13 – 19 1965 | Four Tops - I Can't Help Myself (Sugar Pie, Honey Bunch) | 2 (1) |
| June 20 – 26 1965 | Byrds - Mr. Tambourine Man | 1 |
| June 27 – July 3 1965 | Four Tops - I Can't Help Myself (Sugar Pie, Honey Bunch) | 2 (1) |
| July 4 – 31 1965 | Rolling Stones - (I Can't Get No) Satisfaction | 4 |
| August 1 – 7 1965 | Herman's Hermits - I'm Henry VIII, I Am | 1 |
| August 8 – 28 1965 | Sonny and Cher - I Got You Babe | 3 |
| August 29 – September 18 1965 | Beatles - Help! | 3 |
| September 19 – 25 1965 | Barry McGuire - Eve of Destruction | 1 |
| September 26 – October 2 1965 | McCoys - Hang On Sloopy | 1 |
| October 3 – 30 1965 | Beatles - Yesterday | 4 |
| October 31 – November 13 1965 | Rolling Stones - Get Off Of My Cloud | 2 |
| November 14 – 27 1965 | Supremes - I Hear a Symphony | 2 |
| November 28 – December 18 1965 | Byrds - Turn! Turn! Turn! | 3 |
| December 19 – 25 1965 | Dave Clark Five - Over And Over | 1 |
| December 26 1965 – January 1 1966 | Simon and Garfunkel - The Sounds of Silence | 2 (1) |
28 different songs hit number one in the US during 1965.
Top 5 Songs of 1965

1: Sam The Sham and The Pharaohs - Wooly Bully
2: Four Tops - I Can't Help Myself
3: Rolling Stones - (I Can't Get No) Satisfaction
4: We Five - You Were On My Mind
5: Righteous Brothers - You've Lost That Lovin' Feelin'
2: Four Tops - I Can't Help Myself
3: Rolling Stones - (I Can't Get No) Satisfaction
4: We Five - You Were On My Mind
5: Righteous Brothers - You've Lost That Lovin' Feelin'