This lists songs that hit number one on the charts in the US during 2000. If a song spent nonconsecutive weeks at number one, the number of weeks for each occurrence at the top is shown in parenthesis.
| Date | Artist - Title | Weeks at #1 |
| October 17 1999 – January 8 2000 | Santana - Smooth | 12 |
| January 9 – 22 2000 | Christina Aguilera - What A Girl Wants | 2 |
| January 23 – February 12 2000 | Savage Garden - I Knew I Loved You | 4 (3) |
| February 13 – 19 2000 | Mariah Carey - Thank God I Found You | 1 |
| February 20 – 26 2000 | Savage Garden - I Knew I Loved You | 4 (1) |
| February 27 – March 11 2000 | Lonestar - Amazed | 2 |
| March 12 – April 1 2000 | Destiny's Child - Say My Name | 3 |
| April 2 – June 10 2000 | Santana feat. The Product G&B - Maria Maria | 10 |
| June 11 – 17 2000 | Aaliyah - Try Again | 1 |
| June 18 – July 8 2000 | Enrique Iglesias - Be With You | 3 |
| July 9 – 15 2000 | Vertical Horizon - Everything You Want | 1 |
| July 16 – 22 2000 | Matchbox Twenty - Bent | 1 |
| July 23 – August 5 2000 | 'N Sync - It's Gonna Be Me | 2 |
| August 6 – 19 2000 | Sisqo - Incomplete | 2 |
| August 20 – September 9 2000 | Janet Jackson - Doesn't Really Matter | 3 |
| September 10 – October 7 2000 | Madonna - Music | 4 |
| October 8 – November 4 2000 | Christina Aguilera - Come On Over Baby (All I Want Is You) | 4 |
| November 5 – 11 2000 | Creed - With Arms Wide Open | 1 |
| November 12 2000 – January 27 2001 | Destiny's Child - Independent Women Part 1 | 11 |
18 different songs hit number one in the US during 2000.
Top 5 Songs of 2000
1: Faith Hill - Breathe
2: Santana feat Rob Thomas - Smooth
3: Santana feat The Product G and B - Maria Maria
4: Joe - I Wanna Know
5: Vertical Horizon - Everything You Want
2: Santana feat Rob Thomas - Smooth
3: Santana feat The Product G and B - Maria Maria
4: Joe - I Wanna Know
5: Vertical Horizon - Everything You Want